生命科学学院名校名师讲坛:中组部“千人计划”专家学术报告

北京pk拾赢彩专家 www.aggop.com 来源:生命科学学院发稿时间:2018-05-15 浏览次数:22

报告题目:Structure and functions of G-quadruplex unfolding helicases

报 告 人:奚绪光,博士生导师,中组部“千人计划”特聘教授。

报告时间:2018年5月15日12:00-13:30

报告地点:博C-107教室

个人简介:

 

  奚绪光,博士生导师,中组部“千人计划”特聘教授。主要研究领域:生物大分子的结构及功能。长期以来研究维持染色体组稳定性的生物大分子的结构与功能,特别是以生物物理和结构生物学手段研究解旋酶的作用机理。率先在国际上开展了解旋酶结构及功能的研究,近年来的主要研究方向G-quadruplex解旋酶的结构与分子作用机理,成功运用单分子技术揭示解旋酶与DNA相互反应的新特性。先后发表SCI论著69篇。

学习经历:

1978.9 - 1983.7吉林大学生物化学学士 Bachelor

1986.9 - 1987.7法国巴黎南大学University of Paris-Sud

蛋白质科学Protein Science硕士 Master

1987.9 - 1991.7法国巴黎南大学University of Paris-Sud

蛋白质科学Protein Science博士 Ph.D

工作经历:

1999.7 – 2011.5法国巴黎南大学University of Paris-Sud蛋白质科学Protein Science博导 Habilitation

1983.9 - 1985.7吉林农业大学助教

1991.1 - 1992.12法国国家科研中心 Centre National de la Recherche Scientifique (CNRS)助理研究员Research assistant

1992.1 - 1994.12法国国家科研中心 Centre National de la Recherche Scientifique研究员Researcher

1995.1 - 1996.12法国国家科研中心 Centre National de la Recherche Scientifique一级研究员 Senior Researcher

1998.1 - 2006.12美国国家健康中心 NIH, Washington DC-Bethesda

访问学Visiting Scientist

2006.1 - 2011.12法国科研中心 居里研究所CNRS-Institute Curie

主任研究员Director of Research

2004.1 - 2011.12华东师范大学客座教授兼职

1999.1 - 2011.12中科院物理所客座研究员兼职

2005.1 - 2010.12华东师范大学紫江学者教授兼职

2001.1 - 2011.12西北农林科技大学兼职教授兼职

2008.1 - 2010.12贵州大学客座教授兼职

2011.6 - 今西北农林科技大学“千人计划”特聘教授

  

“G-quadruplex resolving helicases and genome stability” publications (2013-2018)

1.Molecular Mechanistic Insights into Drosophila DHX36-Mediated G-Quadruplex Unfolding: A Structure-Based Model.Chen WF, Rety S, Guo HL, Dai YX, Wu WQ, Liu NN, Auguin D, Liu QW, Hou XM, Dou SX, Xi XG. Structure. 2018 Mar 6;26(3):403-415.e4. doi: 10.1016/j.str.2018.01.008.[IF= 4.945]

2.Helicase Stepping Investigated with One-Nucleotide Resolution Fluorescence Resonance Energy Transfer. Lin W, Ma J, Nong D, Xu C, Zhang B, Li J, Jia Q, Dou S, Ye F, Xi X, Lu Y, Li M. Phys Rev Lett. 2017 Sep 29;119(13):138102. doi: 10.1103/PhysRevLett.119.138102. [IF= 8.80]

3.Insights into the structural and mechanistic basis of multifunctional S. cerevisiae Pif1p helicase.Lu KY, Chen WF, Rety S, Liu NN, Wu WQ, Dai YX, Li D, Ma HY, Dou SX, Xi XG. Nucleic Acids Res. 2018 Feb 16;46(3):1486-1500. doi: 10.1093/nar/gkx1217.

[IF= 10.162]

4.Escherichia coli DNA polymerase I can disrupt G-quadruplex structures during DNA replication. Teng FY, Hou XM, Fan SH, Rety S, Dou SX, Xi XG. FEBS J. 2017 Dec;284(23):4051-4065. doi: 10.1111/febs.14290. Epub 2017 Nov 8.[IF= 3.902]

5.Involvement of G-triplex and G-hairpin in the multi-pathway folding of human telomeric G-quadruplex. Hou XM, Fu YB, Wu WQ, Wang L, Teng FY, Xie P, Wang PY, Xi XG. Nucleic Acids Res. 2017 Nov 2;45(19):11401-11412. doi: 10.1093/nar/gkx766.[IF= 10.162]

6.Effects of monovalent cations on folding kinetics of G-quadruplexes. You J, Li H, Lu XM, Li W, Wang PY, Dou SX, Xi XG. Biosci Rep. 2017 Jul 12;37(4). pii: BSR20170771. doi: 10.1042/BSR20170771. [IF= 2.87]

7.Purification and enzymatic characterization of Gallus gallus BLM helicase. Shi J, Liu NN, Yang YT, Xi XG. J Biochem. 2017 Sep 1;162(3):183-191. [IF= 2.40]

8.Single-molecule studies reveal reciprocating of WRN helicase core along ssDNA during DNA unwinding.Wu WQ, Hou XM, Zhang B, Fossé P, René B, Mauffret O, Li M, Dou SX, Xi XG. Sci Rep. 2017 Mar 7;7:43954. doi: 10.1038/srep43954.[IF= 5.228]

9.A helical bundle in the N-terminal domain of the BLM helicase mediates dimer and potentially hexamer formation. Shi J, Chen WF, Zhang B, Fan SH, Ai X, Liu NN, Rety S, Xi XG. J Biol Chem. 2017 Apr 7;292(14):5909-5920. [IF= 4.125]

10.A 3'-5' exonuclease activity embedded in the helicase core domain of Candida albicans Pif1 helicase.Wei XB, Zhang B, Bazeille N, Yu Y, Liu NN, René B, Mauffret O, Xi XG. Sci Rep. 2017 Feb 20;7:42865. doi: 10.1038/srep42865.[IF= 5.228]

11.AvrXa27 binding influences unwinding of the double-stranded DNA in the UPT box. Zhao J, Zhang B, Jiang J, Liu N, Wei Q, Xi X, Fu J. Biochem Biophys Res Commun. 2017 Mar 4;484(2):390-395. doi: 10.1016/j.bbrc.2017.01.130.[IF= 1.99]

12.Iterative homology checking and non-uniform stepping during RecA-mediated strand exchange. Zhang YW, Nong DG, Dou SX, Li W, Yan Y, Xi XG, Xu CH, Li M. Biochem Biophys Res Commun. 2016 Sep 23;478(3):1153-7. [IF= 1.99]

13.G-quadruplex and G-rich sequence stimulate Pif1p-catalyzed downstream duplex DNA unwinding through reducing waiting time at ss/dsDNA junction. Zhang B, Wu WQ, Liu NN, Duan XL, Li M, Dou SX, Hou XM, Xi XG. Nucleic Acids Res. 2016 Sep 30;44(17):8385-94. doi: 10.1093/nar/gkw669. Epub 2016 Jul 28.[IF= 10.162]

14.Mechanistic insight into cadmium-induced inactivation of the Bloom protein. Qin W, Bazeille N, Henry E, Zhang B, Deprez E, Xi XG. Sci Rep. 2016 May 19;6:26225. doi: 10.1038/srep26225.[IF= 5.228]

15.Pif1 is a force-regulated helicase. Li JH, Lin WX, Zhang B, Nong DG, Ju HP, Ma JB, Xu CH, Ye FF, Xi XG, Li M, Lu Y, Dou SX. Nucleic Acids Res. 2016 May 19;44(9):4330-9. doi: 10.1093/nar/gkw295. [IF= 10.162]

16.Crystal structures of the BsPif1 helicase reveal that a major movement of the 2B SH3 domain is required for DNA unwinding. Chen WF, Dai YX, Duan XL, Liu NN, Shi W, Li N, Li M, Dou SX, Dong YH, Rety S, Xi XG. Nucleic Acids Res. 2016 Apr 7;44(6):2949-61. doi: 10.1093/nar/gkw033. [IF= 10.162]

17.The Bacteroides sp. 3_1_23 Pif1 protein is a multifunctional helicase. Liu NN, Duan XL, Ai X, Yang YT, Li M, Dou SX, Rety S, Deprez E, Xi XG. Nucleic Acids Res. 2015 Oct 15;43(18):8942-54. doi: 10.1093/nar/gkv916.[IF= 10.162]

18.BLM unfolds G-quadruplexes in different structural environments through different mechanisms. Wu WQ, Hou XM, Li M, Dou SX, Xi XG. Nucleic Acids Res. 2015 May 19;43(9):4614-26. doi: 10.1093/nar/gkv361.[IF= 10.162]

19.Unwinding forward and sliding back: an intermittent unwinding mode of the BLM helicase. Wang S, Qin W, Li JH, Lu Y, Lu KY, Nong DG, Dou SX, Xu CH, Xi XG, Li M. Nucleic Acids Res. 2015 Apr 20;43(7):3736-46. doi: 10.1093/nar/gkv209. [IF= 10.162]

20.G-quadruplexes significantly stimulate Pif1 helicase-catalyzed duplex DNA unwinding. Duan XL, Liu NN, Yang YT, Li HH, Li M, Dou SX, Xi XG. J Biol Chem. 2015 Mar 20;290(12):7722-35. doi: 10.1074/jbc.M114.628008. [IF= 4.258]

21.Molecular mechanism of G-quadruplex unwinding helicase: sequential and repetitive unfolding of G-quadruplex by Pif1 helicase. Hou XM, Wu WQ, Duan XL, Liu NN, Li HH, Fu J, Dou SX, Li M, Xi XG. Biochem J. 2015 Feb 15;466(1):189-99. doi: 10.1042/BJ20140997.[IF= 3.797]

22.北京pk拾赢彩专家 Qin W, Liu NN, Wang L, Zhou M, Ren H, Bugnard E, Liu JL, Zhang LH, Vend?me J, Hu JS, Xi XG. J Bacteriol. 2014 Dec;196(24):4216-28. doi: 10.1128/JB.06367-11. [IF= 3.380]

23.Direct measurement of sequential folding pathway and energy landscape of human telomeric G-quadruplex structures. Li W, Hou XM, Wang PY, Xi XG, Li M. J Am Chem Soc. 2013 May 1;135(17):6423-6. doi: 10.1021/ja4019176. [IF= 13.858]